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Non-destructive Analysis (NDA)

Circuit Edit (CKT)

Materials Analysis (MA)

Application Forms

Software

MA-tek FTP

CSR Report

Micro and Nano Biomedical

For nanomedicine, MA-tek adopts appropriate sample preparation protocols (such as vacuum-dried, negative staining, cryo, liquid (K-kit), ultramicrotome, etc) for different types of samples such as virus, vaccine, and nanoformulated agents, etc.  Comprehensive information of their physicochemical parameters can be obtained.  

 


Direct observation in liquid

 


With advanced sample preparation techniques

 

  • Liquid sample TEM (K-kit):A microchip-based holder to contain and seal liquid sample inside for direct TEM observation
  • PLGA:poly (Lactic-co-glycolic Acid)
  • Resovist®:an iron oxide nanoparticle-based MRI contrast agent,(Ferucarbotran)
  • MSNs: Mesoporous Silica Nanoparticles

 

 

TEM Image Analysis

Nanoformulation agents | The first figure shows gold nanoparticles distribution in cells, and the second figure shows nanoparticle morphology.

 

Vaccine | The first figure is the viral particle morphology, and the second figure is the adjuvant. 

 

 

 

Application

A Doxorubicin Encapsulated Liposomes

The images of the liposomes enclosed with drug of Adoxorubincin. (Original condition)

 

Cryo-TEM

UA Negative Stain

 

After an ultrasonic process at 37C for 2 hours, the liposomes were fractured to release the drug. (Leakage condition)

 

 

The size/size distribution of liposome was counted and measured by cryo-TEM images. 

Counted # : original cond. by 403ea, leakage cond. by 225ea     

 

 

NOAAs of abrasives in CMP slurry

K-kit can be used for characterizing the primary and secondary particles in undiluted CMP slurry.

 

 

 

 

 

Dynamic observation of NOAAs in liquid

Dynamic observation of silicate nanoparticles in water

 

Dynamic observation of polystyrene beads under various media

 

 

Nanomaterials & Chemicals

Carbon nanotubes (WMCNT) in Water

Al2O3 nanoparticles in NMP solution

 

Pigment nanoparticles of printer ink

 

 

 

Biological specimens & Nanopharmaceuticals

The nucleoid of E.coli

Collagen bundles in liquid

 

Extracellular vesicles of platelets

Abraxane® drugs (paclitaxel) in saline

 

AuroVist®  gold nanoparticles in PBS 

Liposomes with negative staining 

 

 

Gold nanoparticles (AuNP) in rat blood

K-kits allow direct observation of nanoparticles in blood. Simply place K-kit channel in contact with whole blood sample without any pretreatment, serum will be loaded into the channel by capillary effect.

  ( Anal. Chem. 2012, 84: 6312 −6316 )

 

Image-based statistic analysis of Particle Concentration  (K-kit vs. ICP-MS)

 

Image-based statistic analysis of aggregation and agglomeration of AuNPs in blood

 

 

Micella in aqueous solution

A micelle or micella (plural micelles or micellae, respectively) is an aggregate (or supramolecular assembly) of surfactant molecules dispersed in a liquid colloid. A typical micelle in aqueous solution forms an aggregate with the hydrophilic "head" regions in contact with surrounding solvent, sequestering the hydrophobic single-tail regions in the micelle center.

K-kit can be used for characterizing micelle particles in liquid, by imaging the particle morphology, size and size distribution.  

 

To evaluate the result of micelles in liquid by using K-kit (with liquid), compared with the conventional way by Cu grid (without liquid).

  • Test sample:Micelles solution with particle sizes of around 30-50nm
  • K-kit:Gap0.2um/ SiN100nm ones in wet mode 
  • TEM:Hitachi HT7700 @100KV 

 

In K-kit (Magn. x 10K)

On Cu grid (Magn. x 10K)

There’re many single-micelles that with sizes of around 30nm spread uniformly and only a very few amounts of lumpy objects found in K-kit. By using K-kit, one may characterizes the micelle particles in liquid, without the drying effect to result in aggregates or agglomerates.

 

In K-kit (Magn. x 20K)

On Cu grid (Magn. x 20K)

On the Cu grid, most of the micelles aggregated due to drying effect and only a very few single-micelles remained sporadically. 

The sizes of those single-micelles, which observed of around 10nm, on Cu grid were much smaller than the ones in K-kit, due to the structure collapsed by drying.

 

 

 

MA-tek follows the international regulatory guidance and international standards to provide professional and high quality services on nano biomedical characterization/analysis.